PEQLAB Ltd. • 25 Barnes Wallis Road • Fareham, PO15 5TT
Phone +44(0)1489 889 823 • Fax +44(0)1489 660 040
E-Mail: info@peqlab.co.uk • Web: http://www.peqlab.co.uk
Phone +44(0)1489 889 823 • Fax +44(0)1489 660 040
E-Mail: info@peqlab.co.uk • Web: http://www.peqlab.co.uk
Uracil-DNA-Glycosylase
After incorporation of uracil into the DNA strand, UDG catalyses the hydrolysis of the n-glycosidic bond between uracil and deoxyribose. Before starting the PCR an incubation at 37 °C for 10 minutes degrades uracil containing DNA fragments from previous experiments. The enzyme is deactivated by heat shock at 95 °C for 10 minutes.
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peqlab.co.uk • Web: www.peqlab.co.uk