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PEQLAB Ltd.
PEQLAB Ltd.  •  25 Barnes Wallis Road  •  Fareham, PO15 5TT
Phone +44(0)1489 889 823  •  Fax +44(0)1489 660 040
E-Mail: info@peqlab.co.uk  •  Web: http://www.peqlab.co.uk

Reverse Transcription

 
PEQLAB offers four different enzymes for cDNA synthesis.

peqGOLD AMV Reverse Transcriptase has its temperature optimum at 42 °C and is still working at temperatures up to 58 °C making it suitable for transcribing of RNA templates with strong secondary structures.

peqGOLD M-MULV Reverse Transcriptase (RNase H-, Del) has been genetically modified to remove the associated RNase H activity through deletion. It is suitable for preparation of long DNAs (> 5000 b).

peqGOLD M-MULV (RNase H-, Point Mutant) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long RNA templates (>5000 b). M-MLV RT (H-, Point Mutant) gives higher yields of cDNA compared to the deletion mutant.

peqGOLD M-MULV (RNase H+) can be used in almost all cDNA synthesis applications. However, with long cDNAs RNase H may cause degradation of template RNA. The RNase H activity is weaker than the usually used Avian Myeloblastosis Virus (AMV) reverse transcriptase.


1373.966796875   | Ladezeit bei ISDN etwa 03:21 Minuten.Catalogue Chapter 01 PCR [PDF]

Filesize 1.34 MB


Total 5 products |  Page(s)   1 

 
Cat. No.
Description
 
Price Pound

03-1010
 
03-1011
 
03-1020
 
03-1040
 
03-1041
 
Total 5 products |  Page(s)   1 
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