PEQLAB Ltd. • Bldg. 34, Universal Marina • Crableck Lane • Sarisbury Green, SO31 7ZN
Phone +44(0)1489 578 127 • Fax +44(0)1489 660 040
E-Mail: info@peqlab.co.uk • Web: http://www.peqlab.co.uk
Phone +44(0)1489 578 127 • Fax +44(0)1489 660 040
E-Mail: info@peqlab.co.uk • Web: http://www.peqlab.co.uk
Reverse Transcription
The peqGOLD M-MuLV Reverse Transcriptases possess
RNA- and DNA-dependent polymerase activity. They are genetically
modified and differ from wildtype M-MuLV RT by its structure, catalytic
properties and in the optimum activity temperature. Activity is optimal
at 42 °C and hence above wildtype enzyme. Elevated temperatures reduce
effects of secondary template structure. For first strand cDNA synthesis
up to 13 kb. The enzymes incorporate modified nucleotides (e.g. Cy3,
Cy5, fluorescein) Two version are available:
- peqGOLD M-MuLV H plus RT with RNase H activity which is significantly lower than that of Avian Myeloblastis Virus (AMV) RT.
- peqGOLD M-MuLV H minus RT without RNase H activity and therefore high yields of full-length cDNA from long templates are obtained.
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Price GBP
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peqlab.co.uk • Web: www.peqlab.co.uk